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Mediators of Inflammation
Volume 2014 (2014), Article ID 376518, 9 pages
http://dx.doi.org/10.1155/2014/376518
Research Article

The Antioxidant Profiles, Lysosomal and Membrane Enzymes Activity in Patients with Acute Pancreatitis

1Department of Biomedical and Environmental Analysis, Medical University of Wroclaw, Borowska 211, 50-556 Wroclaw, Poland
2Department and Clinic of Gastrointestinal and General Surgery, Medical University of Wroclaw, M. Curie-Skłodowskiej 66, 50-369 Wroclaw, Poland

Received 20 June 2014; Revised 19 August 2014; Accepted 19 August 2014; Published 15 September 2014

Academic Editor: Yung-Hsiang Chen

Copyright © 2014 Halina Milnerowicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Oxidative stress and inflammatory mediators, such as IL-6, play an important role in the pathophysiology of acute pancreatitis. The study was aimed to assess the degree of the pro/antioxidative imbalance and estimate which antioxidant plays a role in the maintenance of pro/antioxidative balance during acute pancreatitis. The study was investigated in the blood of 32 patients with acute pancreatitis and 37 healthy subjects. IL-6 concentration as early marker of inflammation was determinated. The intensity of oxidative stress was assessed by TBARS concentration. To investigate antioxidative status, the GPx and Cu/Zn SOD activities and the levels of GSH, MT, SH groups, and TRAP were measured. The concentrations of Cu and Zn as ions participating in the maintenance of antioxidant enzymes stability and playing a role in the course of disease were determinated. The activities of GGT, AAP, NAG, and β-GD as markers of tissue damage were also measured. An increase in IL-6 concentration, which correlated with Ranson criteria, and an increase in GPx activity, levels of MT, TBARS, or GGT, and NAG activities in patients group compared to healthy subjects were demonstrated. A decrease in GSH level in patients group compared to control group was noted. The studies suggest that GPx/GSH and MT play the role of the first line of defence against oxidative stress and pro/antioxidant imbalance in the course of acute pancreatitis.