MicroRNA-146a Decreases High Glucose/Thrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression
Figure 1
Endothelial Nox4 expression and intracellular ROS production were significantly induced by HG/thrombin. (a) HAECs were stimulated with HG (25 mmol/L), L-glucose (25 mmol/L), and thrombin (2 U/mL) for 5 h. Real-time PCR showed that stimulation of HAECs with D-glucose/thrombin induced a 10.69-fold synergistic increase of Nox4 mRNA levels compared with the control, while L-glucose/thrombin had no such effect. The bars represent the means ± SEM from 3 experiments. , as compared with the control. , as compared with the control. (b) Stimulation of HAECs with HG/thrombin for 12 h increased Nox4 protein expression 1.35-fold compared with the control; alternatively, L-glucose/thrombin had no discernible effect on Nox4 expression. The bars represent the means ± SEM from 4 experiments. , as compared with the control. (c) Stimulation of HAECs with HG/thrombin for 5 h significantly increased ROS production 2.81-fold compared with the control. The bars represent the means ± SEM from 3 experiments. , as compared with the control. , as compared with the control.
