Roles of 5-Lipoxygenase and Cysteinyl-Leukotriene Type 1 Receptors in the Hematological Response to Allergen Challenge and Its Prevention by Diethylcarbamazine in a Murine Model of Asthma

Figure 4

Response to DEC in vivo in PAS and ALOX mice sensitized and challenged with OVA. PAS and ALOX mice were sensitized to OVA by two injections 14 days apart in (a) and 7 days apart in (b). DEC (12 mg/kg, 200 μL volume) was given orally in water (vehicle, VEIC), 2 h before challenge. Mice were challenged (a) by the i.n. route (3x, 25 μg/25 μL) and in (b) by aerosol (1x, 2,5% grade II OVA/PBS 1x, for 1 h). Bone-marrow was harvested 24 h after the last challenge. Data (mean + SEM) show the numbers of EPO+ cells in freshly harvested bone-marrow from (a) i.n. challenged mice (PAS /9/5/8; ALOX /7/8/8); aerosol-challenged mice (PAS /5/4; ALOX /5/5). All significant differences are shown.

(a)

(b)