Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages
Figure 2
Effect of lancemaside A on the activation of RAW264.7 cells during LPS exposure and on the viability of RAW264.7 cells. ((a) and (c)) The NO level in the culture supernatant of RAW264.7 cells treated with LPS for 24 h (a) or with SNP for 20 min (b) was analyzed by Griess assay. (c) The viability of RAW264.7 cells treated with lancemaside A was determined by MTT assay. (d) The level of iNOS mRNA in RAW264.7 cells treated with lancemaside A (0 to 30 μM) in the presence or absence of LPS (1 μg/mL) for 6 h was determined by real-time quantitative RT-PCR. (e) The surface level of CD80 in RAW264.7 cells treated with LPS for 12 h was determined by flow cytometric analysis. (f) Images of the cells in culture at 12 h were obtained with an inverted phase contrast microscope that was interfaced with a video camera and NIH image software. MFI: mean fluorescence intensity; and versus control.
