Lancemaside A from Codonopsis lanceolata Modulates the Inflammatory Responses Mediated by Monocytes and Macrophages
Figure 7
Effect of BAY11-7082 (BAY) on the inflammatory responses of U937 and RAW264.7 cells. (a) The NO level in the culture supernatant of RAW264.7 cells treated with LPS for 24 h was analyzed by Griess assay. (b) The effect of BAY11-7082 on cell-fibronectin (FN) adhesion was examined with U937 cells that were pretreated with BAY11-7082 and seeded on FN (50 μg/mL) coated plates for 4 h. The number of attached cells was determined by crystal violet staining. (c) RAW264.7 cells were preincubated with BAY11-7082 and then treated with FITC-dextran (1 mg/mL) for 20 min. The level of dextran uptake was determined by flow cytometric analysis. (d) RAW264.7 cells were preincubated with BAY11-7082 and then treated with DHR123 (20 μM) in the presence or absence of SNP (0.25 mM) for 20 min. The level of intracellular ROS was determined by flow cytometric analysis. (e) The viability of RAW264.7 cells treated with BAY11-7082 was determined by MTT assay. MFI: mean fluorescence intensity. versus control.
