PMA Induces Vaccine Adjuvant Activity by the Modulation of TLR Signaling Pathway
Figure 4
Action mechanism of PMA on TLR signaling pathway. (a) PMA induced nuclear translocation of NF-B p65 subunit. HEK293T cells were transfected with phTLR5 for 1 day and treated with or without PMA (10 ng/mL) for 6 hours. The cells were fixed, permeabilized, and immunostained with a polyclonal antibody against NF-B p65, followed by Alexa Fluor-488-conjugated anti-rabbit-IgG antibody. PMA induced nuclear translocation of NF-B p65 subunit in HEK293T cells. (b) PMA induced NF-B activation through PKC-dependent pathway. HEK293T cells transfected with pNF-B-SEAP and phTLR5 plasmids were treated with PMA (10 ng/mL) in the presence of pharmacological inhibitors. PMA-induced NF-B activity was blocked by the treatment of a PKC inhibitor (GF109203X) and an IB inhibitor (Bay11-7082). Wortmannin (WM), Bay 11–7082 (Bay), Genistein (Gen), GF109203X (GF), PD98059 (PD), SB203580 (SB), SP600125 (SP), and U-73122. The data indicate the mean and SEM from three experiments (∗∗).
