Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Antibody </td><td align="center">Type of antibody</td><td align="center">Dilution </td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">CD4</td><td align="center">Monoclonal mouse anti-human<br/>CD4 DAKO cytomation clone 4B12</td><td align="center">1 : 40</td></tr><tr><td align="left">CD8</td><td align="center">Monoclonal mouse anti-human<br/>CD8 DAKO cytomation clone C8/144B</td><td align="center">1 : 50</td></tr><tr><td align="left">CD20</td><td align="center">Monoclonal mouse anti-human<br/>CD20cy DAKO cytomation clone L26</td><td align="center">1 : 200</td></tr><tr><td align="left">CD68</td><td align="center">Monoclonal mouse anti-human<br/>CD68 DAKO cytomation clone PG-M1</td><td align="center">1 : 100</td></tr><tr><td align="left">TGF<i>β</i></td><td align="center">Rabbit polyclonal TGF<i>β</i>1 (V) antibody: sc-146 Santa Cruz Biotechnology</td><td align="center">1 : 50</td></tr><tr><td align="left">FGF<i>β</i></td><td align="center">Rabbit polyclonal FGF-1 antibody (H-125): sc-7910 Santa Cruz Biotechnology</td><td align="center">1 : 100</td></tr><tr><td align="left">COX2</td><td align="center">Monoclonal mouse anti-human<br/>COX-2 DAKO cytomation clone CX-294</td><td align="center">1 : 50</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Antibodies used in the study for immunohistochemical staining.

Mediators of Inflammation

tab1

Table 1

Table 1: Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications 