Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications

Table 2

Immunohistochemical evaluation grading score for CD4/CD8, CD20, and CD68 cells and FGF-β, COX2, and TGF-β of each specimen. (a) Expression of the examined markers in the control group, (b) expression of the markers in the group of mild Graves’ orbitopathy, and (c) expression of the markers in the group of severe Graves’ orbitopathy.

(a)

Number	Classification	CD4/CD8	CD20	CD68	COX-2	FGF	TGF
1	Contrl.	0-0	0	1	0	0	0
2	Contrl.	0-0	0	0	0	0	0
3	Contrl.	0-0	0	0	0	0	0
4	Contrl.	0-0	0	0	0	0	0
5	Contrl	0-0	0	0	0	0	0
6	Contrl	0-0	0	1	0	1	0
7	Contrl.	0-1	1	1	0	1	0

(b)

Number	Classification	CAS score	CD4/CD8	CD20	CD68	COX-2	FGF	TGF
1	Mild GO	1	0-0	0	1	0	1	1
2	Mild GO	3	1-0	0	2	0	2	1
3	Mild GO	2	1-0	0	1	0	1	1
4	Mild GO	3	1-0	0	2	0	2	1
5	Mild GO	1	1-0	0	1	0	1	1
6	Mild GO	3	1-1	0	2	0	1	1
7	Mild GO	1	1-1	0	1	0	1	1
8	Mild GO	3	0-0	0	2	0	1	2

(c)

Number	Classification	CAS score	CD4/CD8	CD20	CD68	COX-2	FGF	TGF
1	Severe GO	10	1-0	0	2	0	2	2
2	Severe GO	10	1-0	0	2	0	2	2
3	Severe GO	9	1-0	0	2	0	2	1
4	Severe GO	8	1-0	0	2	0	2	2
5	Severe GO	7	1-0	0	1	0	1	1
6	Severe GO	10	1-0	0	2	0	2	2
7	Severe GO	10	1-0	0	2	0	2	2
8	Severe GO	9	1-0	2	2	0	2	2
9	Severe GO	10	1-1	0	2	0	2	2
10	Severe GO	10	2-1	0	2	0	2	2
11	Severe GO	5	1-0	0	1	0	1	1
12	Severe GO	10	1-0	0	2	0	2	2
13	Severe GO	10	1-0	0	2	0	2	2
14	Severe GO	6	1-0	0	2	0	1	1
15	Severe GO	5	0-0	0	1	0	1	1
16	Severe GO	5	0-0	0	1	0	1	1
17	Severe GO	5	1-0	0	1	0	1	1
18	Severe GO	8	1-0	0	1	0	2	1
19	Severe GO	10	1-0	0	2	0	2	2
20	Severe GO	7	1-0	0	2	0	1	2
21	Severe GO	5	1-0	0	1	0	2	2
22	Severe GO	10	1-0	0	2	0	2	2

Legend for Tables 2(a), 2(b), and 2(c): immunohistochemistry score:
0-less than 10% positive in 10 representative high power fields (HPF).
1-10%–50% positive cells in 10 HPF.
2-more than 50% positive cells in 10 HPF.
Immunohistochemistry was done using DAB chromogen (brown staining). Ctrl: controls.