5-Azacytidine Promotes an Inhibitory T-Cell Phenotype and Impairs Immune Mediated Antileukemic Activity

Figure 6

Treatment with 5-Azacytidine reduces long-term memory cell phenotype. (a) CD3+ were treated for 48 h with 5 𝜇M 5-Aza or untreated as control. Thereafter, T-cells were first assessed for their CD4 and CD8 expression. CD4+ T-cells as well as CD8+ T-cells were further analyzed for the expression of CD62L and CD127. High expression (>10⁴ compared to isotype control) of both antigens was taken as a surrogate marker for long-term memory cells. A representative example for three independent experiments is shown. (b) CD3+ T-cells were analyzed for the expression of CD62L by flow cytometry after treatment with 5-Aza. Data were shown as mean value of 3 independent experiments with standard deviation. (^∗𝑃<0.05; ^∗∗𝑃<0.01).

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