Vitamin C Mitigates Oxidative Stress and Tumor Necrosis Factor-Alpha in Severe Community-Acquired Pneumonia and LPS-Induced Macrophages
Figure 2
Oxidative stress, DNA damage, TNF-α, and IL-6 in severe CAP. (a) Intracellular ROS were detected by confocal microscope using DHE probe. ROS level was represented by fluorescence intensity (×400). (b) DNA damage was detected by comet assay using confocal microscope (×400). DNA damage score was analyzed with comet assay IV software. (c) SOD activity in serum was measured using the SOD assay kit WST. (d) Correlations between ROS and DNA damage were also evaluated. (e) Correlations between ROS and SOD were also evaluated. Spearman’s correlation with a two-tailed test was used for statistical analyses. (f) Standard ELISA was performed to determine the levels of TNF-α and IL-6.
