Vitamin C Mitigates Oxidative Stress and Tumor Necrosis Factor-Alpha in Severe Community-Acquired Pneumonia and LPS-Induced Macrophages
Figure 5
The effect of vitamin C on LPS-induced autophagy in macrophages. (a) MH-S cells were transfected with RFP-LC3 or GFP-LC3 plasmids for 24 hours. MH-S cells were cultured in the presence or absence of vitamin C and treated with LPS for 12 hours. (b) Western blotting of Beclin1; (c) western blotting of LC3. β-actin was probed as a loading control in (b) and (c).
