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Mediators of Inflammation
Volume 2014, Article ID 436438, 11 pages
http://dx.doi.org/10.1155/2014/436438
Research Article

Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae

1Division of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USA
2Department of Pathology and Laboratory Medicine, University of California, Irvine, CA 92697, USA

Received 12 May 2014; Revised 6 August 2014; Accepted 7 August 2014; Published 1 September 2014

Academic Editor: Alex Kleinjan

Copyright © 2014 Sangeetha Prakash et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The optimal concentration of CPn for activation of MoDCs was determined by stimulating the MoDCs with varying concentrations of CPn for 24h.Subsequently the DCs were collected and stained for upregulation of surface markers, CD83, CD86 by flow cytometry using specific antibodies. Supernatant collected was assayed for cytokines, TNF-a and IL1b by ELISA. Multiplicity of infection (MOI) 1CPn:1DC was found to be optimal. Bar diagrams depict the surface markers and cytokines in DCs after CPn stimulation. A. CD86; B. CD83; C. TNF-a; D. IL-1b.Figure is mean +/- S.D. of 5 subjects.

  1. Supplementary Material