The Impact of Staphylococcus aureus-Associated Molecular Patterns on Staphylococcal Superantigen-Induced Toxic Shock Syndrome and Pneumonia
Figure 1
Modulation of expression of HLA-DR3 and CD86 on CD11b+ cells by HKSA. Splenic mononuclear cells from HLA-DR3 transgenic mice were cultured with medium, HKSA (108 bacteria/mL), SEB (1 μg/mL), or SEB + HKSA. 24 hours later, the cells were harvested and washed and expression of HLA-DR3 and CD86 on CD11b+ cells was analyzed by flow cytometry. Mononuclear cells were first gated based on forward and side scatter profiles. Other analyses were performed on cells within this gate. Representative histogram profiles and bar charts depicting median fluorescent intensity (MFI) are given. Each bar represents mean ± SE from 2 different experiments, each performed in triplicate. compared to cells cultured with medium.
