The Impact of Staphylococcus aureus-Associated Molecular Patterns on Staphylococcal Superantigen-Induced Toxic Shock Syndrome and Pneumonia

Table 1

Modulation of SEB-induced TSS by staphylococcal PAMPs.

HLA class II type	Treatment^a	D-gal	Mortality
HLA-DR3	None	+	0/4
HLA-DR3	PGN	+	0/4
HLA-DR3	HKSA	+	0/4
HLA-DR3	SEB (10 μg)	+	8/8
HLA-DR3	SEB (5 μg)	+	6/6
HLA-DR3	SEB (10 μg) + PGN	+	5/5
HLA-DR3	SEB (5 μg) + PGN	+	4/4
HLA-DR3	SEB (10 μg) + HKSA	+	3/3
HLA-DR3	SEB (5 μg) + HKSA	+	4/4
HLA-DQ8	None	+	0/4
HLA-DQ8	PGN	+	0/4
HLA-DQ8	HKSA	+	0/4
HLA-DQ8	SEB	+	6/6
HLA-DQ8	SEB (10 μg) + PGN	+	6/6
HLA-DQ8	SEB (10 μg) + HKSA	+	6/6

HLA-DR3 and HLA-DQ8 transgenic mice were pretreated with D-galactosamine and an hour later challenged with the following agents. Animals were followed for 9 hours.
^aTreatments given
PGN: peptidoglycan—50 μg/mouse
HKSA: heat-killed S. aureus—10⁸ bacteria/mouse
SEB: staphylococcal enterotoxin B—10 μg/mouse or 5 μg/mouse
D-gal: D-galactosamine—30 mg/mouse 1 hour prior to being challenged with PGN, HKSA, SEB or at the same time.