Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation

<table>LPS causes upregulation of PRDX1<i> in vitro.</i> (a) Morphological changes in BEAS-2B cells after treatment with 1 <i>μ</i>g/mL or 10 <i>μ</i>g/mL LPS in 12 hours or 24 hours. BEAS-2B without any treatment was used as a control, original magnification, ×200. (b) Effects of LPS on cell viability. Results are presented as the percentage absorbance of the control group. Data are expressed as the means ± SD. <svg height="17.825001" id="M54" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 69.150002 17.825001" width="69.150002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus the control group. (c) and (d) the expression of PRDX1 in culture media was evaluated by western blotting (c) and ELISA (d) after challenge with LPS. <i>α</i>-Tubulin was not detected in the conditioned media. Experiments in (c) and (d) were repeated at least 3 times, with similar results. Each bar represents the mean ± SD of three independent experiments.<svg height="17.825001" id="M55" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 69.150002 17.825001" width="69.150002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>.</table>

Mediators of Inflammation

fig4

Figure 4

Figure 4: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation 