Chemokine CXCL16 Expression Suppresses Migration and Invasiveness and Induces Apoptosis in Breast Cancer Cells
Figure 3
Knockdown of CXCL16 increases migration and invasion, but not proliferation, of MCF-7 cells. MCF-7 cells were treated with lipofectamine alone (mock) or infected with negative plasmids (sh NC) or CXCL16 targeted sequence (Sh CXCL16). 48 h later cells were collected and underwent migration, invasion, and proliferation assays. (a) ShRNA effectively inhibited expression of CXCL16 mRNA ((a1), qRT-PCR), total CXCL16 protein ((a2), western blotting), and TM-CXCL16 ((a3), flow cytometry). (b) CXCL16 knockdown enhanced migration of MCF-7 cells. compared with mock or negative control. n.s.: nonsignificant compared between mock and negative control. (c) CXCL16 knockdown enhanced invasion of MCF-7 cells. compared with mock or negative control. n.s.: nonsignificant compared between mock and negative control. (d) CXCL16 knockdown had no influence on proliferation of MCF-7 cells. n.s.: nonsignificant compared between mock and negative control (sh NC).
