Consequences of the Lack of CD73 and Prostatic Acid Phosphatase in the Lymphoid Organs
Figure 5
Synergistic effect of PAP and CD73 deficiency on the leukocyte subpopulations. Lymphocyte phenotypes in blood (a), spleen (b), lymph nodes (c), and thymus (d) of wild type mice (), CD73 single (), PAP single (), and CD73/PAP double knockouts () (thymus: for all genotypes) were analyzed using flow cytometry. (e) Regulatory T cells in lymph nodes. Examples of the FACS analyses of regulatory T cells in wild type and CD73/PAP KO mice are shown. In the left panels the gating strategy is shown and in the middle panels percentages of CD4+/CD25+ cells are indicated. Intensities of FoxP3 stainings of CD4+/CD25+ cells are shown as histograms in the right panels. Logarithmic fluorescence is on -axis and the number of cells on -axis. (f) Combined results of regulatory T cell analyses of different organs are shown as indicated. , , and .001.
