Herbal Extract SH003 Suppresses Tumor Growth and Metastasis of MDA-MB-231 Breast Cancer Cells by Inhibiting STAT3-IL-6 Signaling

Figure 2

SH003 suppresses tumor growth in vivo. (a) 1×10⁶ MDA-MB-231 cells were s.c. injected and nude mice (𝑛=5/group) were p.o. administrated with the indicatives until 34 days. Xenograft tumor volumes were measured three times a week by a caliper. ^∗𝑃<0.05. (b) Body weights were measured three times a week. (c) Tumor tissues were stained with hematoxylin and eosin. Photo images were taken at 20x magnification. Tumor tissues were also stained with anti-CD31 antibody to detect tumor angiogenic vessels. The bar indicates 10 μm. (d) To measure tumor angiogenic vessels in tumor cohorts, CD31-positive vessels were counted. ^∗𝑃<0.05. (e) Pulmonary metastases were determined by counting foci at lungs.

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