Research Article

Cholesterol Oxidase Binds TLR2 and Modulates Functional Responses of Human Macrophages

Figure 3

Membrane and intracellular location of ChoD in THP-1-derived macrophages. Macrophages were incubated with ChoD (0.5 U/mL) for 24 h and stained with anti-ChoD primary and PE-conjugated secondary antibodies. ChoD was detected on intact cells (membrane location) and in permeabilized cells (intracellular location). Data are means ± SEM of MFI values from six separate experiments ( ) (a). The representative histograms show the fluorescence intensities of intact (b) and permeabilized cells (c).