Research Article

Cholesterol Oxidase Binds TLR2 and Modulates Functional Responses of Human Macrophages

Figure 5

Competition of ChoD and LTA for binding sites on THP-1-derived macrophages. Macrophages were preincubated with or without LTA (10 or 100 μg/mL) and then treated with ChoD (0.5 U/mL or 1 U/mL, (a) or (b), resp.) for 30 min at room temperature. After treatment, cells were stained as described in Section 2, and MFI values were measured by flow cytometry. Cells that were not treated with LTA or ChoD, but were stained with antibodies, were used for examination of nonspecific binding. The values for nonspecific binding were subtracted from the corresponding sample values. Data are means ± SEM of MFI values from three separate experiments ( , , for LTA-untreated macrophages versus LTA-treated macrophages; Student -test).