Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes
Figure 5
Lycopene-induced modulation of IL-8 and IL-1 gene expression in Caco2 cells. (a) Lycopene induced upregulation of basal and LPS-induced IL-8 gene expression in Caco2 cells. IL-8 gene expression was studied in Caco2 cells treated with LPS. Modulation of this expression was studied in Caco2 cell cultures pretreated for 2 h with or without vectorized lycopene. Then, according to assays, LPS (100 ng/mL) was added for 6 h and mRNA expression was analyzed by RT-qPCR. (b) Lycopene induced no modification of gene expression induced by THP1-derived conditioned medium (CM) in Caco2 cells. THP1 cells were or not exposed to PMA for 2 hours. Then, the cell culture medium was discarded and the cells were extensively washed and a fresh medium without PMA was added and culture was resumed for 6 h. At the end of the culture period, the conditioned media (CM) were collected and then added to Caco2 cell cultures pretreated or not with lycopene for 12 h. The final treatment of Caco2 cells with the conditioned medium (CM) was for 6 h and, finally, expression of inflammatory genes was assessed by qRT-PCR. (c) Lycopene downregulates IL-1 gene expression induced by THP1-derived conditioned medium (CM) in Caco2 cells. THP1 cells were or not exposed to PMA for 2 hours. Then, the cell culture medium was discarded and the cells were extensively washed and a fresh medium without PMA was added and culture was resumed for 6 h. At the end of the culture period, the conditioned media (CM) were collected and then added to Caco2 cell cultures pretreated or not with lycopene for 12 h. The final treatment of Caco2 cells with the conditioned medium (CM) was for 6 h and, finally, expression of inflammatory genes was assessed by qRT-PCR. Values are means SD (). Assays treated with lycopene were compared to corresponding assays without lycopene. ; ; , nonsignificantly different.
