Ticlopidine in Its Prodrug Form Is a Selective Inhibitor of Human NTPDase1

Figure 1

Influence of ticlopidine on intact HUVEC and COS-7 cells transfected with an expression vector encoding human NTPDase1. The substrate (ATP or ADP) was added together with ticlopidine, both at 100 μM. Relative activities are expressed as the mean ± SD of 3 independent experiments with confluent cells (HUVEC from three different donors at passage 2), each performed in triplicate; mean cell number in one well was in the order of 250,000. The activity (without ticlopidine, which was set at 100%) with the substrate ATP corresponded to and  nmol min⁻¹· for HUVEC and transfected COS-7 cells, respectively, and with ADP to and  nmol min⁻¹· for HUVEC and transfected COS-7, respectively. Data are presented as the mean ± SD of 3 experiments carried out in triplicate. ; ; .