Sphingosine-1-phosphate/S1P Receptors Signaling Modulates Cell Migration in Human Bone Marrow-Derived Mesenchymal Stem Cells
Figure 6
The essential role of the Rho pathway in S1PR2-mediated suppression of human BMSCs migration. (a) Left: cells were stimulated with S1P (1 μM) for the indicated time. Right: cells were pretreated with S1PRs antagonists (S1PR1 antagonist W146, S1PR2 antagonist JTE-013, and S1PR3 antagonist CAY1444) and then were stimulated with 1 μM S1P for 5 min. RhoA activities were determined by a pull-down assay. (b) Serum-starved BMSCs were pretreated with the indicated concentration of Y27632 and then were allowed to migrate for 4 h in the presence of 1 nM S1P.
