Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro

Figure 2

The Th1 response was suppressed by anti-IL-17A mAb and IL-1Ra treatments. ((a) and (b)) The secretions of IFN-𝛾 and IL-2 in supernatant of the macrophage-lymphocyte cocultured system were assayed by ELISA. ((c), (d), and (e)) The expressions of IFN-𝛾, IL-2, and T-bet in lymphocytes were assayed by real-time PCR. The concentration of IL-1Ra is 15 μg/mL and the concentration of anti-IL-17A mAb is 20 μg/mL. Data were presented as mean ± SEM (𝑛=5). #: 𝑃<0.05, significantly different compared with the PBS group. Δ: 𝑃<0.05, significantly different compared with the silica group.

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