Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
Figure 3
Effect of preexposure to IFN-γ on CD40L-induced CCL5 expression and release by HPFB. Cells were pretreated for 48 hours either with control medium or IFN-γ (100 U/mL). After that cells were stimulated with CD40L for the next 24 hours. (a) Expression of mRNA for CD40 and CCL5 was assessed by conventional RT-PCR. Results of a representative experiment of two performed. (b) CCL5 release was measured in HPFB cultures established from 5 separate donors. Asterisks represent a significant difference compared to cells not exposed to IFN-γ.
