Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ

Table 1

Effect of sequential addition of TNF-α and IFN-γ on CCL5 release by HPFB.

Stimulus 1	Stimulus 2	CCL5 (pg/10^4 cells)
Medium	Medium	Undetectable
Medium	IFN-𝛾	Undetectable
Medium	TNF-𝛼	4±2
Medium	TNF-𝛼 + IFN-𝛾	24±13
IFN-𝛾	Medium	1±1
IFN-𝛾	TNF-𝛼	9±4
TNF-𝛼	Medium	6±4
TNF-𝛼	IFN-𝛾	9±7
TNF-𝛼 + IFN-𝛾	Medium	146±20

Cells were incubated with TNF-𝛼 (1000 pg/mL) and/or IFN-𝛾 (25 U/mL) for 24 hours (stimulus 1), washed, and incubated again for the next 24 hours in the presence or absence of these cytokines (stimulus 2). Data were derived from two independent experiments.