Mediators of Inflammation / 2014 / Article / Fig 2

Research Article

Aryl Hydrocarbon Receptor (AhR) Modulates Cockroach Allergen-Induced Immune Responses through Active TGFβ1 Release

Figure 2

AhR expression in CRE-treated human lung fibroblasts. (a) AhR expression in WI-38 was detected using antibody against AhR (red line) and IgG2 (blue line) by flow cytometry. (b-c) Fibroblasts were treated with different doses of TCDD (0.1 nM and 1 nM) for 2 to 48 hours; expressions of cyp1a1 (b) and cyp1b1 (c) were examined by RT-PCR. (d) Fibroblasts were treated with 50 μg/mL CRE for 2–48 hours and AhR expression was examined by RT-PCR. (e) Immunofluorescence analysis of AhR expression in CRE-treated fibroblasts at various doses (0–100 μg/mL). Bars represent mean ± SEM of 3 independent experiments.

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