Mediators of Inflammation / 2014 / Article / Fig 5

Research Article

Aryl Hydrocarbon Receptor (AhR) Modulates Cockroach Allergen-Induced Immune Responses through Active TGFβ1 Release

Figure 5

AhR controls fibroblast differentiation induced by CRE. (a) Differentiation of fibroblasts with and without AhR knockdown was evaluated by the expression of α-SMA with DAPI for nuclei immune-staining after cells were treated with CRE (50 μg/mL) for 24 and 72 hours. (b) Positive staining for α-SMA was analyzed by Imaging software (iVision; Biovision). (c) α-SMA expression was detected by RT-PCR in fibroblasts with or without AhR knockdown. Bars represent mean ± SEM of 3 independent experiments, ** . (d) Vimentin was detected by immune-staining after cells were treated with CRE (50 μg/mL) for 72 hours. (e) Both -SMA and vimentin expression were detected by western blots after WI38 cells were treated with 50 μg/mL CRE for 72 h.

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