Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi
Figure 2
The protective immunity elicited by heterologous prime-boost vaccination in susceptible mice. F1 (CB10XBALB/c) male mice were immunized i.m. exactly as described in the legend of Figure 1. Fourteen days later, mice were challenged s.c. with 1,000 trypomastigotes of the indicated parasite strain. (a) Mean parasitemia ± SD of each mouse group () challenged with parasites of the Brazil strain. The values of G2 and G3 mice at days 21, 25 and 35 were lower than those of G1 mice ( in all cases, one-way ANOVA, Tukey HSD). Asterisks denote that the values of G3 mice were lower than those of G2 mice (); (b) The Kaplan-Meier survival curves of the different groups were compared, and the results showed that G3 survived significantly longer () than the two other groups; (c) Mean parasitemia ± SD of each mouse group () challenged with parasites of the Colombian strain. The values of G2 and G3 mice at days 25 and 31 were lower than those of G1 mice ( in all cases). Asterisks denote that the values of G3 mice were lower than those of the G2 mice (); (d) The Kaplan-Meier survival curves of the different groups were compared, and the results showed that G3 survived significantly longer () than the two other groups. The results were obtained from one of two independent experiments.
