Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi
Figure 3
The protective immunity elicited by heterologous prime-boost vaccination in resistant mice. (a) F1 (CB10XBALB/c) female mice were immunized i.m. as depicted. For priming, G2 mice received a mixture of control plasmid pcDNA3 and of pIL-12 (300 μg), and G3 mice were immunized with a mixture of pIL-12, pIgSPCl9 and p154/13 (300 μg). Twenty-one days later, these mice received, in these same spots, 100 μL of a viral suspension containing a total of 2 × 108 pfu of rec. adenovirus. For boosting, G2 mice received control adenovirus Adβgal, and G3 mice were immunized with a mixture of rec. adenovirus AdASP-2 and Ad-TS. Fourteen days later, mice were challenged s.c. with 1,000 trypomastigotes of the indicated parasite strain. (b) Mean parasitemia ± SD of each mouse group () challenged with parasites of the Brazil strain. Asterisk denotes that at day 37, the values of G3 mice were lower than those of G2 mice (, one-way ANOVA, Tukey HSD); (c) Mean parasitemia ± SD of each mouse group () challenged with parasites of the Colombian strain. Asterisks denote that at days 13 to 31, the values of G3 mice were lower than those of G2 mice ().
