Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
Figure 6
The involvement of histone acetylation and methylation in the regulatory effect of LLLT on TNF-α expression. The levels of TNF-α expression in LLLT-treated THP-1 cells in the presence or absence of AA (a) and MTA (d) using ELISA assay. ChIP analysis of the relative levels of acetylated H3 (AcH3) (b), acetylated H4 (AcH4) (c), and trimethylated H3K4 (e) at the TNF-α gene locus. ChIP encompassed the following subregions relative to the transcription start site: TNF1 (T1, +99 to –42), TNF2 (T2, +32 to –119), TNF3 (T3, –100 to –250), TNF4 (T4, –195 to –345), +1417, +720, and –1700. The relative levels were normalized to the input DNA and shown as the means (SD) of 3 individual study subjects. was considered significant compared with the control without inhibitor.
