Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification

Figure 7

LLLT-induced IP-10 expression in human monocytes via inhibiting histone acetylation, but not trimethylation. The levels of IP-10 expression in LLLT-treated THP-1 cells in the presence or absence of AA (a) and MTA (d) using ELISA assay. ChIP analyses of the relative levels of (b) acetylated H3 (AcH3) and (c) acetylated H4 (AcH4) at the IP-10 gene locus encompassing the following subregions relative to the transcription start site: CXCL10/IP-10-1: +9 to −172; CXCL10/IP-10-2: −444 to −622. The relative levels were normalized to the input DNAs and shown as mean (±SD) of 3 individual study subjects. ^∗∗𝑃<0.01 was considered significant versus without the addition of inhibitor.

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