Involvement of Different CD4⁺ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens

Figure 2

Immune response induced by LmES in total PBMCs or PBMCs depleted of CD4⁺ or CD8⁺ T cells. Whole PBMCs or those depleted of CD4⁺ (-CD4) or CD8⁺ T cells (-CD8) from 4 HZCL (◆) and 5 HC (◊) were stimulated with LmES (10 μg/mL) for 5 days. (a) Proliferative responses were assessed by [³H]-thymidine uptake. IFN-γ (b) and GrB (c) secretion was evaluated in supernatants of cultures at day 5 using ELISA tests. Bars represent median values. Statistical analysis: ^∗𝑃<0.05, when comparing the stimulated cells to the unstimulated cells, (Unst) and ^∗∗𝑃<0.05, when comparing HZCL to HC.

(a)

(b)

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