Involvement of Different CD4⁺ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens

Figure 5

Both CD4⁺CD25⁺ and CD4⁺CD25⁻ T cell subsets produce IFN-γ and GrB in response to LmES in vitro. T cell subsets CD4⁺CD25⁺ and CD4⁺CD25⁻ were purified from the peripheral blood of 4 HZCL and 3 HC. The cells were stimulated with LmES in vitro in presence of mitomycin C-treated autologous PBMCs (APCs) at different Effector/APC ratios for 5 days. Culture supernatants were used to determine IFN-γ (a) and GrB (b) concentrations using ELISA tests. Results are expressed as the mean ± standard error of the mean of levels. Statistical analysis: ^∗𝑃<0.05 when comparing HZCL to HC.