Involvement of Different CD4⁺ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens

Figure 6

LmES induce GrB production by CD4⁺CD25⁺CD127^dim/− regulatory T in HZCL. CD4⁺CD25⁺CD127^dim/− regulatory T cells were purified from peripheral blood of 4 HZCL and 3 HC and stimulated with LmES (10 μg/mL) in vitro in the presence of mitomycin C-treated PBMCs (APCs) at different Effector/APC ratios for 5 days. Culture supernatants were used to determine GrB concentration. Results are expressed as the mean ± standard error of the mean of levels. Statistical analysis: ^∗𝑃<0.05 when comparing HZCL to HC.