TLR2 Elicits IL-17-Mediated RANKL Expression, IL-17, and OPG Production in Neutrophils from Arthritic Mice

Figure 4

Methylated H3K9 (methyl H3) in nuclear extracts from neutrophils. Ly6G⁺CD11b⁺ cells were purified and stimulated with (20 μg/mL) or/and IL-17 (40 ng/mL) for 10 min. Nuclear extracts were obtained as described in Section 2. Methylated H3K9 in nuclear extracts from neutrophils was detected by immunoblot (IB). Lamin in nuclear extracts was used as a control for protein content. Densitometry analyses of IBs were performed by ImageJ 1.42 software (NIH, Bethesda, MD, USA). The density of methyl H3K9 lines was normalized to lamin expression in each sample and was presented in units.