Paeonol Attenuates Cigarette Smoke-Induced Lung Inflammation by Inhibiting ROS-Sensitive Inflammatory Signaling

Figure 6

Paeonol (Pae) attenuates increases in extracellular and intracellular levels of reactive oxygen species (ROS) induced by cigarette smoke extract (CSE) in human bronchial epithelial cells. Cells were exposed to medium alone or 3% CSE for 2 (a) and 30 minutes (b) with pretreatment with N-acetyl-cysteine (NAC, 2 mM; a ROS scavenger), Pae (0.4 mM), or the vehicle of Pae. After exposure, the culture medium was removed for the measurement of extracellular ROS levels (a). The cells were collected for the measurement of intracellular ROS levels (b). Levels of ROS were measured by HE/ETH fluorescent probe assay. Data in each group are mean ± SEM from four independent experiments. ^∗𝑃<0.05 versus control. ^#𝑃<0.05 versus CSE without drug pretreatment.

(a)

(b)