Mediators of Inflammation / 2014 / Article / Tab 1 / Research Article
The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence Table 1 Primers used for real-time PCR.
Gene Primer sequence (5′-3′) Fragment size, bp GenBank accession number LPAR1 ACGGAATCGGGATACCATGA 86 NM_174047.2 CCAGTCCAGGAGTCCAGCAG LPAR2 TTCTATGTGAGGCGGCGAGT 161 NM_001192235.1 AGACCATCCAGGAGCAGCAC LPAR3 TCCAACCTCATGGCCTTCTT 101 NM_001192741.2 GACCCACTCGTATGCGGAGA LPAR4 CCACAGTACCTCCAGAAAGTTCA 192 NM_001098105.1 TTGGAATTGGAAGTCAATGAATC ATX ACCCCCTGATTGTCGATGTG 120 NM_001080293.1 TCTCCGCATCTGTCCTTGGT PLA2 CTGCGTGCCACAAAAGTGAC 92 NM_001075864.1 TCGGGGGTTGAAGAGATGAA FST GCAGCTCTACATGCGTGGTG 133 NM_175801.2 TGACAGGCACTGGGGTAGGT GDF9 TCGGACATCGGTATGGCTCT 86 NM_174681.2 GGATGGTCTTGGCACTGAGG BMP15 GCAGAGGAAGCCTCGGATCT 104 NM_001031752.1 CAATGGTGCGGTTTTCCCTA CTSB GGCTCACCCTCTCCAGTCCT 136 NM_174031.2 TCACAACCGCCTTGTCTGAA CTSK GAACCACTTGGGGGACATGA 77 NM_001034435.1 GGGAACGAGAAGCGGGTACT CTSS CCGCCGTCAGCATTCTTAGT 99 NM_001033615.1 CATGTGCCATTGCAGAGGAG CTSZ GGGGAGGGAGAAGATGATGG 146 NM_001077835.1 CCACGGAGACGATGTGGTTT BCL2 GAGTTCGGAGGGGTCATGTG 203 NM_001166486.1 GCCTTCAGAGACAGCCAGGA BAX GTGCCCGAGTTGATCAGGAC 126 NM_173894.1 CCATGTGGGTGTCCCAAAGT GAPDH CACCCTCAAGATTGTCAGCA 103 NM_001034034.2 GGTCATAAGTCCCTCCACGA
