Lysophosphatidic Acid Signaling in Late Cleavage and Blastocyst Stage Bovine Embryos

Table 1

Target genes and primer pair sequences used in qRT-PCR.

Gene	Primer sequence (5′-3′)	Fragment size, bp	GenBank accession number
LPAR1	ACGGAATCGGGATACCATGA; CCAGTCCAGGAGTCCAGCAG	86	NM_174047.2
LPAR2	TTCTATGTGAGGCGGCGAGT; AGACCATCCAGGAGCAGCAC	161	NM_001192235.1
LPAR3	TCCAACCTCATGGCCTTCTT; GACCCACTCGTATGCGGAGA	101	NM_001192741.2
LPAR4	CCACAGTACCTCCAGAAAGTTCA; TTGGAATTGGAAGTCAATGAATC	192	NM_001098105.1
ATX	ACCCCCTGATTGTCGATGTG; TCTCCGCATCTGTCCTTGGT	120	NM_001080293.1
PLA2	CTGCGTGCCACAAAAGTGAC; TCGGGGGTTGAAGAGATGAA	92	NM_001075864.1
BCL2	GAGTTCGGAGGGGTCATGTG; GCCTTCAGAGACAGCCAGGA	203	NM_001166486.1
BAX	GTGCCCGAGTTGATCAGGAC; CCATGTGGGTGTCCCAAAGT	126	NM_173894.1
3βHSD	TCCCGGATGAGCCTTCCTAT; ACTAGGTGGCGGTTGAAGCA	116	NM_174343.2
COX-2	TGGGTGTGAAAGGGAGGAAA; AAGTGCTGGGCAAAGAATGC	127	AF004944.1
PTGES	CCGAGGACGCTCAGAGACAT; AAAGCCCAGGAACAGGAAGG	122	NM_174443.2
PGFS	GGAGGACCCCAGGATCAAAG; CTCAGCAATGCGTTCAGGTG	130	S54973.1
IGF2R	ACCTCCGATCCTCAATCCCA; TGTAGTTGAAGTGCCGGTCC	89	NM_174352.2
OCT4	GAGAAAGACGTGGTCCGAGTG; GACCCAGCAGCCTCAAAATC	101	NM_174580.2
GAPDH	CACCCTCAAGATTGTCAGCA; GGTCATAAGTCCCTCCACGA	103	NM_001034034.2