Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study

Figure 4

Early CL and mid-CL explants in vitro production of P₄ ((a) and (b)); PGE₂ ((c) and (d)); and PGF_2α ((e) and (f)), after 24 h treatment with no exogenous factor = Control or with GHR (50 ng/mL), LEP (5 ng/mL), LEP (200 ng/mL), LEP (5 ng/mL)+GHR (50 ng/mL), LEP (200 ng/mL)+GHR (50 ng/mL), or LH (positive control; 10 ng/mL). Bars represent mean ± SEM. Different letters indicate significant differences (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001). Correspondent value of control for hormone production mean ± SEM: early CL P₄ (1.05±0.031 ng/mg); mid-CL P₄ (6.305±0.30 ng/mg) and for PGE₂ (0.23±0.05 ng/mg) and PGF_2α (0.147±0.015 ng/mg).

(a)

(b)

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(f)