Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study

Figure 5

Early CL and mid-CL explants in vitro angiogenic activity, assessed after bovine aortic endothelial cell (BAEC) viability measurement ((a) and (b)) and secretion of migration inhibitory factor (MIF, (c) and (d)); tumor necrosis factor α (TNF, (e) and (f)); and nitrite ((g) and (h)), after 24 h treatment with no exogenous factor = Control or with GHR (50 ng/mL), LEP (5 ng/mL), LEP (200 ng/mL), LEP (5 ng/mL)+GHR (50 ng/mL), or LEP (200 ng/mL)+GHR (50 ng/mL). Bars represent mean ± SEM. Different asterisks indicate significant differences (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001). Correspondent values of control for hormone production mean ± SEM: TNF (0.24±0.009 ng/40 mg); MIF (27.47±0.75 ng/mg); and nitrite (0.18±0.037 M/mg).

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