Overexpression and Selectively Regulatory Roles of IL-23/IL-17 Axis in the Lesions of Oral Lichen Planus

Table 2

Primer design for real-time quantitative PCR.

Gene	GeneBank accession number	Primer sequences	PCR product size (bp)
IL-17	NM_002190.2	Forward: 5′-atgactcctgggaagacctcat-3′	150
		Reverse: 5′-gttcaggttgaccatcacagtc-3′
IL-23p19	NM_016584.2	Forward: 5′-ccttctctgctccctgatagc-3′	118
		Reverse: 5′-gactgaggcttggaatctgct-3′
IL12p40	NM_002187.2	Forward: 5′-ctggagaaatggtggtcctca-3′	113
		Reverse: 5′-gacttggatggtcagggttttg-3′
β-Defensin-1	NM_005218.3	Forward: 5′-ccttctgctgtttactctctgc-3′	126
		Reverse: 5′-gaatagagacattgccctccac-3′
β-Defensin-2	NM_004942.2	Forward: 5′-gggtcttgtatctcctcttctcg-3′	130
		Reverse: 5′-ctagggcaaaagactggatgac-3′
β-Defensin-3	NM_001081551.2	Forward: 5′-ccaggtcatggaggaatcat-3′	113
		Reverse: 5′-gagcacttgccgatctgttc-3′
CXCL-9	NM_002416.1	Forward: 5′-gggactatccacctacaatcctt-3′	127
		Reverse: 5′-ctgctgaatctgggtttagacat-3′
CXCL-10	NM_001565.3	Forward: 5′-gctgtacctgcatcagcattagt-3′	138
		Reverse: 5′-gacatctcttctcacccttctttt-3′
CXCL-11	NM_005409.4	Forward: 5′-ttgtgtgctacagttgttcaagg-3′	110
		Reverse: 5′-atggaggctttctcaatatctgc-3′
CCL-5	NM_002985.2	Forward: 5′-catattcctcggacaccacac-3′	131
		Reverse: 5′-ctttcgggtgacaaagacgac-3′
CCL-20	NM_004591.2	Forward: 5′-tgtgctgtaccaagagtttgctc-3′	124
		Reverse: 5′-tgaagaatacggtctgtgtatccaa-3′
IL-8	NM_000584.3	Forward: 5′-agctctgtgtgaaggtgcagtt-3′	126
		Reverse: 5′-ggtccactctcaatcactctcag-3′
IL-6	NM_000600.3	Forward: 5′-ggagacttgcctggtgaaaatc-3′	140
		Reverse: 5′-gcaggaactggatcaggactt-3′
TNF-α	NM_000594.3	Forward: 5′-aagcctgtagcccatgttgtag-3′	112
		Reverse: 5′-gctggttatctctcagctccac-3′
GAPDH	NM_002046.4	Forward: 5′-ctttggtatcgtggaaggactc-3′	132
		Reverse: 5′-gtagaggcagggatgatgttct-3′