Troglitazone and Δ2Troglitazone Enhance Adiponectin Expression in Monocytes/Macrophages through the AMP-Activated Protein Kinase Pathway
Figure 5
TG and Δ2TG enhanced AMPK phosphorylation. Macrophages were treated with 9 μM of TG or Δ2TG for the indicated time ((a), (d)) or with the indicated concentration of TG or Δ2TG for 45 min ((b), (e)). ((c), (e)) Macrophages were incubated for 1 h with compound C (an AMPK inhibitor) and then for 45 min with or without 9 μM TG or Δ2TG in the continued presence of the inhibitor, and then, the phosphorylated AMPK expression was measured in cell lysates by Western blotting. AMPK was used as the loading control. as compared to the untreated cells. as compared to the TG or Δ2TG-treated cells.
