Troglitazone and Δ2Troglitazone Enhance Adiponectin Expression in Monocytes/Macrophages through the AMP-Activated Protein Kinase Pathway

Figure 7

TG and Δ2TG reduced the adhesion of THP-1 cells to TNF-α-treated HUVECs. HUVECs were pretreated for 4 h with 3 ng/mL of TNF-α. THP-1 cells were left untreated or were pretreated for 1 h with 0.2 μg/mL of purified antiadiponectin antibody (Ab-ADI) and then with 9 μM TG or with Δ2TG for 18 h. In addition, THP-1 cells were left untreated or were pretreated for 1 h with 5 μM GW9662 (GW) or 0.625 μM compound C (Com C) and then with 9 μM TG or with Δ2TG for 18 h in the continued presence of the inhibitor. The BCECF/AM-labeled THP-1 cells were added to TNF-α-treated HUVECs in a 24-well plate and incubated for 1 h, and then the nonadherent cells were removed by two gentle washes with PBS and the number of bound monocytes counted by fluorescence microscopy. N represents HUVECs without any treatment. C represents HUVECs with TNF-α treatment. as compared to the C cells. as compared to TG-treated cells and Δ2TG-treated cells, respectively. Bar = 100 μm.