Anti-Inflammatory Functions of Protein C Require RAGE and ICAM-1 in a Stimulus-Dependent Manner
Figure 1
Effect of PC on leukocyte recruitment in wild-type, RAGE , and Icam-1 mice during trauma-induced inflammation. Leukocyte adhesion (number of adherent cells per mm2 of surface area) in cremaster muscle venules of wild-type (WT) control mice, RAGE mice, and Icam-1 mice was recorded with and without treatment with PC (100 U/kg, 3 hours) during trauma-induced inflammation (a). Leukocyte adhesion in the same genotypes and treatment groups was shown after additional stimulation with fMLP (superfusion at 1 μM, 15 min) (b). Leukocyte transmigration (per mm2 surface area) was analyzed in Giemsa-stained cremaster muscle whole mounts after 15 min fMLP superfusion (1 μM) in the trauma model in WT, Icam-1 , and RAGE mice with and without PC treatment (100 U/kg, 3 hours) (c). All values are presented as mean ± SEM from three or more mice per group. Significant differences () to WT and RAGE control mice are indicated by the asterisks and pound key, respectively.
