Anti-Inflammatory Functions of Protein C Require RAGE and ICAM-1 in a Stimulus-Dependent Manner
Figure 2
Effect of PC on leukocyte recruitment in wild-type, RAGE , and Icam-1 mice after TNFα stimulation. Leukocyte adhesion (number of adherent cells per mm2 of surface area) in 3 h TNFα-stimulated (500 ng/mouse) cremaster muscle venules of wild-type (WT) control mice and Icam-1 and RAGE mice was documented with and without treatment with PC (100 U/kg, 3 hours) (a). Leukocyte adhesion in the same genotypes and treatment groups was depicted after additional stimulation with fMLP (superfusion at 1 μM, 5 min) (b). Leukocyte transmigration (per mm2 surface area) was analyzed in Giemsa-stained cremaster muscle whole mounts after 5 min fMLP superfusion (1 μM) in the TNFα-model in WT, Icam-1 , and RAGE mice with and without PC treatment (100 U/kg, 3 hours) (c). All values are presented as mean ± SEM from three or more mice per group. Significant differences () to WT and Icam-1 control mice are indicated by the asterisks and pound key, respectively.
