Mediators of Inflammation

Research Article

Polymorphisms in the Tumor Necrosis Factor Receptor Genes Affect the Expression Levels of Membrane-Bound Type I and Type II Receptors

Table 2

The level of membrane-bound TNFα types I and II receptors expressed on CD3⁺, CD19⁺, or CD14⁺ PBMC subpopulations.


	Percent of cells expressing receptors		Number of receptors per cell
	TNFRI	TNFRII	TNFRI	TNFRII

CD19⁺ cells	1.3 (0.9–2.1)*	7.9 (5.8–12.3)*	1153.7 (891.9–1490.8)*	1102.5 (933.4–1309.2)*
CD3⁺ cells	1.5 (0.9–2.6)*	36.6 (28.4–47.6)**	427.3 (349.2–524.7)**	570.3 (516.8–627.1)**
CD14⁺ cells	11.4 (4.0–28.8)	28.3 (12.2–43.3)***	869.4 (756.9–1017.9)***	1273.9 (1053.0–1450.6)***
Mock-stimulated CD14⁺ cells¹	9.8 (5.8–35.0)	50.0 (31.2–72.1)^†	1267.5 (1053.0–1521.1)^†	1983.4 (1677.9–2268.9)^†
LPS-stimulated CD14⁺ cells¹	9.6 (4.0–32.3)	79.2 (71.6–87.8)	1718.2 (1245.0–2559.1)	4177.0 (3133.1–5173.5)

Data are expressed as median values (interquartile range).
¹Mononuclear cells (2 × 10⁶/mL) were cultured in the absence or presence of LPS (E. coli serotype 055:B5) at a concentration of 200 ng/mL for 24 h. *Significantly different from CD14⁺ cells (); **significantly different from CD19⁺ and CD14⁺ cells (); ***significantly different from CD14⁺ cells harvested from mock-stimulated PBMCs (); ^†significantly different from CD14⁺ cells harvested from LPS-stimulated PBMCs ().