Figure 7: Effect of RGSF-A on cytokine production profile in cultured and anti-CII peptide stimulated splenocytes isolated from CIA mice. Splenocytes from naïve, RGSF-A, MTX, or vehicle-treated mice isolated, cultured, and collagen peptide stimulated followed by analysis of cytokine levels in the supernatant as indicated in the materials and methods section. Treatment of RGSF-A at higher dose reduced CIA IL-17 (a) and IL-6 (b) secretions but induced IL-10 production in splenocytes from CIA mice cultured with anti-CII peptide. , , and as compared with vehicle treatment; for each group.