Involvement of IL-13 and Tissue Transglutaminase in Liver Granuloma and Fibrosis after Schistosoma japonicum Infection

Figure 2

IL-13 level was associated with hepatic fibrosis after Sj infection. (a, b) IL-13 mRNA level in BALB/c mice liver is upregulated at weeks 5, 6, 8, and 12 after Sj infection, as determined through RT-PCR and SYBR Green based Q-PCR. GAPDH is detected as an internal control. Data are presented as mean ± SD from eight mice per group. ^∗𝑃<0.05 and ^∗∗𝑃<0.01. (c) Concentration of IL-13 in mice sera is tested by ELISA. Data are presented as mean ± SD from eight mice per group. ^∗𝑃<0.05 and ^∗∗𝑃<0.01. (d) Localization and expression level of IL-13 protein in BALB/c mice liver is shown through IHC assay at weeks 5, 6, 8, and 12 after Sj infection. Representative IL-13 staining sections are shown at primary 40x magnification.

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