Innate Immunity to Leishmania Infection: Within Phagocytes
Figure 1
Infection with L. major reduces production of NO and IL-12 and increases expression of M2 marker LIGHT by differentiated macrophages. (a-b) Bone marrow derived macrophages (BMDM from B6 mice) were infected or not with L. major after 6 days. Next day, all BMDM were treated with IFN-γ, and next day, some of the BMDM cultures were treated with IL-4. (a) After 3 d, cells were washed and restimulated with medium (control) or LPS. After 2 additional days, the levels of nitrites, IL-12p40, and TNF-α were determined. (b) After 3 d, expression of LIGHT was evaluated by cellular ELISA. Results are expressed as mean and SE of triplicates (a) or quadruplicates (b), , .
